Создание генно-инженерного штамма-продуцента шикимовой кислоты на основе бактерий рода bacillus

Abstract

Shikimic acid(SA) is a key chiral starting molecule for the synthesis of the neuramidase inhibitor GS4104 against viral influenza(the only one effective clinic drug against avian influenza). It is a keymetabolic intermediate of the shikimate pathway for biosynthesis of aromatic amino acids(L-Phe, L-Trp, and L-Tyr) and many alkaloids in plants and microorganisms. As a commercial product, SA has been extracted from the fruits of the Illicium plant. However, microbial fermentation as an alternative process for SA production has attracted mor and more interests. In this paper, functions of key enzymes in the biosynthesis pathway of shikimic acid were analyzed, and the breeding of engineering bacterium by genetic improvements for high yield of shikimic acid was also discussed. Knockout SA kinase gene(aroK) in genome of Bacilus.Subtilis, with the method of homologous recombination.