Isolation and identification of promising strains of bacillus subtilis
Bibliographic entry
Mirzabaev, B. M. Isolation and identification of promising strains of bacillus subtilis / B. M. Mirzabaev, A. Y. Sergazina, A. K. Mukhtarov // Информационные технологии в образовании, науке и производстве [Электронный ресурс] : материалы научно-технической интернет-конференции, Минск, 21-22 ноября 2022 г. / сост. М. Г. Карасёва. – Минск : БНТУ, 2023. – С. 171-175.
Abstract
The article presents data on the isolation and study of the physiological and biochemical properties of Bacillus subtilis cultures. Samples of Chernozem soils of wheat fields were used as materials for selecting crops. 10 isolates with antimicrobial properties of B. subtilis were isolated from the samples. Microscopic observation of these isolates showed that they are gram-positive, rodshaped, endospore-forming bacteria. According to the cultural-morphological and physiological-biochemical characteristics, the isolated isolates were identified as Bacillus subtilis. Work was carried out to study the antagonistic activity of isolated Bacillus subtilis strains to 2 test strains: Alternaria alternata and Fusarium avenaceum. Based on the data obtained, it was noted that Bacillus subtilis strains EU7, EU14, EU22, EU31 and EU34 have antagonistic activity against Alternaria alternata and Fusarium avenaceum. Thus, promising strains for biocontrol of alternarious and Fusarium diseases were obtained.